We are given a purified preparation of a plasmid that bears a certain gene of in

Question

We are given a purified preparation of a plasmid that bears a certain gene of interest. Which of the following experimental procedures might be done using the plasmid WITHOUT introducing it into bacterial cells?

A1. Determining the length of the gene of interest.

A2. Isolating the protein encoded by the gene of interest.

A3. Obtaining larger amounts of the same plasmid DNA.

A4. Testing if the gene of interest improves the fitness of bacteria under UV irradiation.

A5. None of the above require putting the plasmid into cells first.

Explanation / Answer

A1- this can be done without putting plasmid into cells by just doing agarose gel elecrophoresis. As you know the size of plasmid DNA, run the ladder for reference and then determine the size of the cells are transformed then you'll get increased size from that you can deduct the plasmid size without gene of interest and you can get the length of gene of interest.

A2- -To get protein of gene of interest it has to be introduced first into bacterial cells.

A3- obtaining large amount of plasmid DNA can be done without putting plasmid into bacterial cells by doing PCR reactions.

A4- To check the fitness of bacteria under UV irradiation we first need to out plasmid into bacterial cells and then only we can irradiate and see whether the fitness of bacteria improves or not.

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