Probes are short, single-stranded DNA or RNA segments that are used to identify

Question

Probes are short, single-stranded DNA or RNA segments that are used to identify DNA fragments with a particular sequence. In order to identify a specific restriction fragment using a probe, what must be done? A) The fragments must be separated by electrophoresis. B) The fragments must be treated with heat or chemicals to separate the strands of the double helix. C) The probe must be hybridized with the fragment. D) Only A and B are correct. E) A, B, and C are correct DNA fragments from a gel are transferred to a nitrocellulose paper during the procedure called Southern blotting. The purpose of transferring the DNA from a gel to a nitrocellulose paper is to: A) permanently attach the DNA fragments to a substrate. B) separate the two complementary DNA strands. C) transfer only the DNA that is of interest. D) prepare the DNA for digestion with restriction enzymes. E) separate out the PCRs. After being digested with a restrietion enzyme, genomie DNA fragments are separated by gel electrophoresis. Specific fragments can then be identified through the use of a: A) plasmid. B) restriction enzyme. C) sticky end. D) acid probe. E) ligase. Which of these is not one of the procedures used in Southern blotting? A) electrophoresis B) hybridization C) autoradiography D) restriction fragment preparation E) transformation The unpaired nucleotides produced by the action of restriction enzymes are referred to as A) sticky ends. B) base sequences. C) single strands. D) restriction fragments. E) ligases.

Explanation / Answer

Question #21. E) A, B and C are correct. Because, probes are oligonucleotides or polynucleotides, DNA, RNA, single or double-stranded, and natural or modified, either in the nucleotide bases or in the backbone which are useful for detecting a complementary target sequence in a complex nucleic acid mixture.

Question #24. E) transformation. Because, transformation refers to the altering a cell genetically by direct uptake or incorporation of exogenous genetic material from its surroundings through the cell membrane.

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