You might not consider the Midwest when someone mentions a trip to the beach, bu

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You might not consider the Midwest when someone mentions a trip to the beach, but the Lake of the Ozarks is a hotspot in the United States. Centrally located in the heart of Missouri, the lake is a great gathering place for Middle America vacations. It is one of the largest human-created lakes, containing roughly 600 billion gallons of fresh water, spanning approximately 61,000 acres and about 1100 miles of shoreline. Flow of the lake water is controlled by the Bagnell Dam AmerenUE operates the dam for electrical production, in conjunction with the Army Corps of Engineers, which monitors the dam for spill-off, reservoir, and commerce. In a typical year, the lake system boasts 3 5 million tourists from Memorial Day to Labor Day, drawing in millions of dollars in vacations, tourism, boating, golf, hotels, shopping, and entertainment. During the summers of 2009 to 2012, things have been changing at the Lake of the Ozarks. Bacterial growths have been "blossoming' in the water, which have the potential to cause disease. As a result, repeated closures have been necessary, and the beaches have been much quieter. The Missouri Department of Natural Resources (MODNR) routinely the waters for safety by counting fecal coliform bacteria. Fecal coliforms are a collection of microbes that live in large members in the intestines of warm-blooded animals (including humans). The best-known member of this group is Escherichia coli, but not all E. coli fecal coliform strains cause disease. However, E. coli can act as a marker of other microbial pathogen levels in the water. A sample of lake water was added to a petri dish of nutrient agar using the streak plate method and incubated at 38 degree C for 48 hours After 48 hours the petri dish contained white and red colonies and there were no isolated colonies. 1 How was growth on the plate detected? ______ 2 Name the inoculum. _____ 3 Give the incubation parameters. _____ 4 Is this a pure or mixed culture, explain? _____ 5 Give reasons why no isolated colonies were obtained. ______ 6 Name the media._______

Explanation / Answer

1) Growth on the plate was detected by the appearance of bacterial colonies.

2) The inoculum used in this method is a loopful of lake water obtained from the lake of Ozarks

3) Incubation parameters are temperature maintained at 38 degree celcius for 48 hours at neutral pH of 7.4

4) This is a mixed culture because different types of bacterial colonies are obtained.

5) Isolated colonies are not obtained because the culture medium allows growth of all fecal coliformbacteria

6) The culture media used is streak plate method is agar plate.

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