Compounds I, II, and III are in the following biochemical pathway in bacteria Th

Question

Compounds I, II, and III are in the following biochemical pathway in bacteria The biochemical pathway begins with a precursor. Mutation a inactivates enzyme A in the pathway, mutation b inactivates enzyme B, and mutation c inactivates enzyme C. Mutant strains of bacteria, designated mutant a, b, or c, each have one of these defects. The mutant strains of bacteria were tested on minimal medium lo which compound I, II, or III was added The bacteria require compound III in order to grow. Place a plus or a minus in the table to designate if the mutant strain will grow on medium with that compound present. A plus indicates the mutant will grow, whereas a minus indicates that the mutant will not grow.

Explanation / Answer

Mutant strain a enzyme activity for A is loss but other enzymes B and C activity still remains. So in Mutant strain a compound I is added, so anyway compund I will be get for the mutant strain a. It not depends on enzyme A. It grows in all the compounds.

Mutant strain b enzyme activity for B is loss but other enzymes A and C activity still remains. But if we add compound I it not able to convert into compound B. So it not able to grow in that medium.

But if we add compund B it convert in to compound C by enzyme C and it will grow.

Mutant I II III a + + + b - + + c - - +

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