This is for my biochemistry lab, the experiment is dealing with trypsin and BPTI
ID: 946577 • Letter: T
Question
This is for my biochemistry lab, the experiment is dealing with trypsin and BPTI. I need to make a graph: plot the absorbance change per minute versus the BPTI concentration for each cuvette.
here are my cuvettes and amount of BPTI added to each
1- 0uL BPTI added
2- 10 uL BPTI added
3-20 uL BPTI added
4-30 uL BPTI added
5- 40 uL BPTI added
6- 50 uL BPTI added.
Each cuvette has a different amount of water and trypsin added to them, for a total volume of 100 uL in each cuvette.
I was give a sample of BPTI for which I had to find the concentration. Using the absorbance, I calculated that the concentration was 0.035mM. We then had to dilute this 10-fold, so to 0.0035mM.
I am not sure how to find the concentration of BPTI in each cuvette with this information. The molecular weight of BPTI is 6500. I don't know if that is needed.
I feel like this should be really easy, but I am having trouble doing this.
Explanation / Answer
Make a table
S.No.
BPTI (uL)
Water
Trypsin
OD
1.
0
90
10
2.
10
80
10
3.
20
70
10
4.
30
60
10
5.
40
50
10
6.
50
40
10
BPTI molecular weight = 6500
BPTI concentration = 0.035 mM
If it is 10 fold BPTI concentration= 0.0035 mM
C1V1= C2V2
0.035 X 100 = C2 X 10
C2= 0.035 X 100/10
= 0.0035 mM
If it is 10 fold BPTI concentration= 0.00035 mM
C2= 0.035 X 100/20
= 0.175 mM
If it is 10 fold BPTI concentration= 0.0175 mM
C2= 0.035 X 100/30
= 0.00116 mM
If it is 10 fold BPTI concentration= 0.000116 mM
C2= 0.035 X 100/40
= 0.875 mM
If it is 10 fold BPTI concentration= 0.0875 mM
C2= 0.035 X 100/50
= 0.07 mM
If it is 10 fold BPTI concentration= 0.007 mM
S.No.
BPTI (uL)
Water
Trypsin
OD
1.
0
90
10
2.
10
80
10
3.
20
70
10
4.
30
60
10
5.
40
50
10
6.
50
40
10
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