Please help me answer question Medgar Evers College Pr,blem Set 8 Prof Small ) T

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Medgar Evers College Pr,blem Set 8 Prof Small ) The tollowing sequence of DNA is tran uence of DNA is transcribed and encodes a small protein with the sequence Met Tyr Arg Gly Ala. 5-CCTATGCCCGCATCATTACGCACCTCTGTACATGGC-3 3 GGATACGGGCGTAGTAATGCGTGGAGACATGTACCC-5 a) Which strand of DNA is the template strand for transcription? Which direction (right to left or left to right) does RNA polymerase move along the template as it transcribes this gene? b) Whais the sequence of the nucleotides in the processed mRNA molecule for this gene? Indicate the 5' and3' directions of this gene c) A single base mutation in the gene results in synthesis of the peptide Met Tyr. What is the sequence of nucleotides making up the mRNA produced by this mutant gene? List three characteristics of the genetic code. How can you distinguish experimentally among DNA polymerase III, RNA polymerase nase? ppose you isolate a mutant bacterium that does not have an active ligase enzyme ium replicate? Why or why not? At what step will replication be halted?

Explanation / Answer

1. a. The 5'-3' strand is used as the template for RNA production. As transcription moves from 3' to 5', the direction will be right to left.

b. 5'-AUGUACAGAGGU-3'

C. The mutation is in the codon AGA, changing it to UGA, which is a stop codon. The mRNA would read as 5'-AUGUACUGAGGU-3'

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