A portion of the eukaryotic gene encoding the peptide Met-Tyr-Arg-cily-Ala is sh

Question

A portion of the eukaryotic gene encoding the peptide Met-Tyr-Arg-cily-Ala is shown below. 5'....CCCCTATGCCCCCCTGGGGGAGGATCAAAACACTAACCTGTACATGGC.....3' 3'....GGGGATACGGGGGGACCCCCTCCTAGTTTTGTGATTGGACATGTACCG.....5' a. Which DNA strand (top or bottom) is the sense strand? ______ b. Which direction (right to left or left to right) does the RNA polymerase move along the anti-sense strand in order to transcribe this DNA? ______ c. Does this sequence have any introns? If so - how many? Box the intron(s) in. d. What sequence of DNA nucleotides results in the expression of this Met-Tyr-Arg-Gly-Ala peptide? e. A mutation in this gene results in the expression of the peptide Met-Tyr-Ser-Gly-Gly What mutation produced this new peptide and what is the new sequence of DNA nucleotides after this mutation?

Explanation / Answer

Answer:

Based on the given sequence:

a) The coding strand or the sense strand (non transcribed) is the top one (5' to 3') direction.

b) RNA polymerase moves along the anti sense (or template) strand in 3' to 5' direction.

c) Yes this sequence has introns:

d) Met-Tyr-Arg-Gly-Ala peptide (AUG-UAU/UAC) ATG-TAC-AGG-GGT-GCT

Working on other parts.

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