4) How can we determine the effectiveness of the seperation based on TLC separat

Question

4) How can we determine the effectiveness of the seperation based on TLC separation? 5) What are the reasons that could have effects on contaminating samples in TLC? one will be extractd is soldtion containing an organic acid salt and a neutral compound, which one will be extracted into ethyl acetate upon mixing in a separatory fu ve an aqueous solution containing an organic acid salt and a neutral compound, whic n a separatory funneutral compound, which 1. If you ha xing in a separatory funne 2. If an organic compound dissolves in an aqueous base such as NaOH, what does this tell you about the nature of the compound? 3. Y ou are given a beaker of an aqueous solution in which an organic compound has been dissolved. With the aid of pH paper, you estimate that the solution has a pH of 3. Which of the following should you use to precipitate the organic compound out of the aqueous solution? Explain your reasoning. a. water b. HCI c. NaOH d. ethyl acetate

Explanation / Answer

1. Ethylacetate being organic would extract the neutral moelcule in it and the organic acid salt would remain soluble in the aqueous phase.

2. If a compound dissolves on addition of NaOH, an acidic group such as Phenol OH is present in the system.

3. If the pH measured is 3, it is salt of amine. Amines salt can be reprecipitated by addiiton of NaOH to neutralize the H+ in the molecule.

4. In TLC separation the difference between the spots (compounds) whould atleast be 0.1 and 0.2 Rf value. The solvent front must always be greater than 0.5-0.6 Rf value from the spot to be isolated.

5. Samples in TLC may be contaminated by presence of unwanted acid or base.

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