(Problem 19) Two mutations that affect plaque morphology in phages (a– and b–) h

Question

(Problem 19) Two mutations that affect plaque morphology in phages (a– and b–) have been isolated. Phages carrying both mutations (a– b–) are mixed with wild-type phages (a+ b+) and added to a culture of bacterial cells. Subsequent to infection and lysis, samples of the phage lysate are collected and cultured on bacterial cells. The following numbers of plaques are observed: What is the frequency of recombination between the a and b genes?

A. 42%

B. 44%

C. 14%

D. 7%

E. 0%

Explanation / Answer

Recombinant frequency is calculated by the following formula,

= number of recombinants / total number of offsprings x 100

here the number of recombinants are 320 (a+b-) and 357 (a-b+)

Therefore, the recombinant frequency is

= (320+357)/5854 x 100

= 677/4854 x 100

= 0.1394 x 100

= 13.94%

approximately 14%

Therefore,the answer for this question is option (C) 14%.

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