sapling learning The data at right describe the binding of a ligand to a proteir

Question

sapling learning The data at right describe the binding of a ligand to a proteirn (mM) 0.06 L] is the concentration of free ligand is the fraction of ligand-binding sites on the protein that are occupied by the ligand (Note that some textbooks use terms like v or Y, instead of , to denote fractional saturation.) | 0.10 0.14 0.21 0.37 0.53 0.79 1.11 1.31 1.70 0.08 | 0.12 0.17 0.23 0.36 0.42 0.53 0.58 0.62 0.68 Answer parts (a) and (b), below (a) Which of the following graphs could be used to estimate Kd for the data? B. 1.0 0.9 0.8 0.7 0.6 0.5 O A and E O A and D O B and D O B and E O C and E 0.4 0.3 0.2 0.1 0.0 continued below... 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 (b) Calculate Kd.

Explanation / Answer

Kd is the equilibrium binding constant, in the same units as X. It is the radioligand concentration needed to achieve a half-maximum binding at equilibrium.

Kd= 0.85 mM

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