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Need help answering this question. Phosphorous is required to synthesize the sam

ID: 93721 • Letter: N

Question


Need help answering this question.

Phosphorous is required to synthesize the sample E. coli containing triphosphates used in DNA A of the bacteria is then transferred nonradioactive for many generations to a medium that contains a radioactive isotope of phosphorus (32P). Samples of the bacteria are removed immediately after the transfer and after one and two rounds of replication. Assume that newly synthesized DNA contains P and the original DNA contains nonradioactive phosphorous. What will be the distribution of radioactivity in the DNA of the bacteria in each sample? Immediately after the transfer After one round of replication After two rounds of replication One strand has 50% of the DNA 50% of the DNA molecules Both strands have P incorporated have azP in both strands have 32P in one strand P incorporated into the DNA.

Explanation / Answer

Ans.) The distribution of radioactivity in the DNA of the bacterial in each sample is given below--

Immediately after the transfer -- No, 32P is incorporated.

After one rounds of replication – One strand has 32P   incorporated.

After two rounds of replication -- 50% of the DNA has 32P in both strands and 50% will have 32P in one strand only.

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