Which of the following does not achieve sterilization? Dry heat Pasteurization A
ID: 93704 • Letter: W
Question
Which of the following does not achieve sterilization? Dry heat Pasteurization Autoclave Formaldehyde Ethylene oxide Which of the following is a limitation of the autoclave? Length of time Ability to inactivate viruses Ability to kill endospores Use with heat labile materials Use with glassware Which of the following substances is the least effective antimicrobial agent? Soap Cationic detergents Phenolics Iodine Alcohol Which of the following is not used for the disinfection of water? Ozone Gamma radiation Chlorine Copper sulfate None of the above Alcohols exert their antimicrobial action by denaturing proteins dissolving lipids both a & b formation of hypochlorous acid all of the above The length of time in which 90% of a bacterial population will be killed at a given temperature is the generation time thermal death point thermal death time Decimal reduction time Quaternary ammonium compounds exert their antimicrobial effects by Disrupting tExplanation / Answer
8 Formaldehyde
Formaldehyde is a potentially dangerous chemical, can be toxic, allergenic, and carcinogenic It is considered even more dangerous than ethylene oxide and is therefore less commonly used for sterilization and moreover Formaldehyde has weak penetrating power than ethylene oxide makes sterilization less durable.
9 Use of heat labile materials
Heat labile materials like plastics bottles cannot be sterilizes in autoclave as moist heat of autoclave may caused damage or distortion of materials and it may also limit penetration of moist heat.
10 Soap
Regular soap won’t kill healthy bacteria on the skin’s surface, Soap can lift dirt and oils off the surfaces so that it can be easily rinsed away but not harmful bacteria and germs.
11 Copper sulphate
Copper sulphate may affect the visible algae but it may not permanently remove the nutrient source of algae and once it settle down it may release toxins harmful for human use, slightest contact of copper sulphate with human skin may cause allergic reaction and permanent change of skin colour
12 Alcohol works by denaturing proteins and lipids.
it works by denaturing proteins and dissolving lipids effectively destroying many types of bacterial and viral cells, by dissolving lipid it attack the layer around the viral genome and by denaturing protein it inactivates viral genome and protein making molecules.
13 Thermal death time.
Thermal death time is used to determine how long it takes to kill a specific bacteria a under specific temperature for stipulated time.
14 Disrupting Plasma membrane
Quaternary ammonium compounds bind irreversibly to the phospholipids and proteins of the membrane thereby impairing permeability affecting bacterial cell to absorb nutrients.
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