Linker scanning mutagenesis has been used to analyze the promoter of the tk gene

Question

Linker scanning mutagenesis has been used to analyze the promoter of the tk gene of herpes simplex virus. The results of the study are summarized below, with each “box” representing a linker insertion of 6 to 10 bp.

a.) Based on these results, draw a map showing the position of specific nucleotide regions important for the
efficient transcription of the tk gene.

b.) What type of control element is represented by each of these transcriptionally important regions?

c.) Which of these transcriptionally important regions is likely to contain two neighbouring control elements?

Explanation / Answer

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