How would describe the morphology of Borrelia burdorferi? You are attempting to

Question

How would describe the morphology of Borrelia burdorferi?

You are attempting to determine the number of bacteria in one gram of your garden soil. You prepare a series of dilutions and plate each one. What would you expect your plated results to look like?

What is a colony forming unit (CFU)when looking at the plates?

Comparing the diameter of Bacillus megaterium a measured using a basic stain and acid stain. What would you name this microorganism?

Which stain is use for basic staining technique?

Explanation / Answer

Borrelia burdorferi is an gram negative bacteria, and a spirocheate. They are thin, helical, highly motile and highly wound. They are visible through Giemsa staining. When serial dilutions are done and each one are plated, it is easy to count the number of bacteria because, there will be decrease in the number of bacterial cells, with increase in dilution. For higher dilution there will be minimum number of cells, that are easy to enumertae. Inb this way dilution technique is used to calculate the number of cells. Colony forming unit is the measure of number of viable cells in the plate. Bacillus anthracis can be the other microbe. Hematoxylin and methylene blue can be used as stains in the basic staining.

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