1. Describe an experiment of your design to build upon what was found in this st
ID: 92832 • Letter: 1
Question
1. Describe an experiment of your design to build upon what was found in this study (make sure it is clearly related to the specific findings in this article! You will lose points if it is not closely related to the topic at hand!).
2. Propose two changes you would have made to the experiments described in the article to strengthen THIS publication (these should be realistic changes to the research described, not an entirely different experiments – use that for question #1!)
Veterinary Parasitology 205 (2014) 300-306 Contents lists available at ScienceDirect Veterinary Parasitology ELSEVIER journal homepage www.elsevier.com/locate/vet par Occurrence of Dipylidium caninum in fleas from client-owned Cross Mark cats and dogs in Europe using a new PCR detection assay Frédéric Beugnet a,* Michel Labuschagne b, Josephus Fourie b, Jacques Guillot Robert Farkasd, Vasile Cozma e, Lénaig Halosa, Klaus Hellmann. Martin Knausg, Steffen Rehbeing Merial SA-S, 29 Av Tony Garnier, 69007 lyon, France b ClinVet,Box 86. Universitas, Bloemfontein, 9321, Republic of South Africa Ecole Vétérinaire de Maisons-Alfort, 94704 Maisons-Alfort Cédex, France Szent Istvan University, Faculty of Veterinary Science, Budapest, Hungary Veterinary Faculty, Cluj-Napoca, Romania Klifovet AG, Geyerspergenstr. 27, 80689 Munchen, Germany erial GmbH, Kathrinenhof Research Center, Walchenseestr 8-12, 3101 Rohrdorf Germany ABSTRACT ARTICLE INFO Article history Ctenocephalides fleas are not only the most prevalent ectoparasites of dogs and cats but Received 18 April 2014 also the intermediate host of the cestode Dipylidium caninum. Due to the poor sensitivity Received in revised form 4J une 2014 coproscopy to diagnose cat and dog infestation by Dipylidium, few epidemiological data of Accepted 5 June 2014 are available on its prevalence among pet populations. A new PCR method was developed to specifically identify D caninum rDNAinside single fleas. The PCR test was then applied to Keywords 5529 fleas of Ctenocephalides genus, 2701 Ctenocephalides felis fleas (1969 collected on 435 Dipylidium caninum cats and 732 on 178 dogs) and 2828 Ctenocephalides canis fleas collected from 396 dogs Ctenocephalides felis Precisely, 4.37% of cats were infested by a flea population infected with D. caninum. Out of Ctenocephalides canis he 1969 felis from cats, 2.23% were found to be infected with Dipylidium. From the 396 Cats dogs infested with C. canis, 9.1%% were infested with the Dipylidium infected fleas, which Dogs s significantly higher than the observation made in cats (p 0.03). Moreover, 3.1% of the C canis fleas were found to be infected with Dipylidium, which is not significantly different than in C felis. Looking at the number of infected fleas in the positive samples (at least one PCR pos ve fea in a sample), the infestation rate in samples was varied from 3 to 100% with an average of 19.7% which is in favour of easy and regular Dipylidium reinfestations of both cats and dogs in households. For the first time, the spread of D. caninum between fleas and dogs and cats is confirmed throughout Europe O 2014 Elsevier BV. All rights reserved et al., 2000; Dryden and Rust, 1984; Franc et al., 1998 1. Introduction Mircean et al., 2010; Rust and Dryden, 1997) Neverthe- The cat flea, Ctenocephalides felis, is the main flea species less, the prevalence of Ctenocephalides canis appears to be infesting both dogs and cats (Bond et al., 2007; Cadiergues greater than previously believed in many regions, espe- cially in central and eastern Europe where C canis is the predominant flea species infesting dogs (Xhaxhiu et a 2009: Farkas et al., 2009). Even in areas where C felis felis Corresponding author. Tel.: +0033 687748983 appears to be predominant, the prevalence of canis in dog fax: +0033 472723298 populations may still vary from 10 to 12.5% like in France E-mail address: frederic beugnet@merial.com (F. Beugnet. http://dx.doi.org/10.1016/j.wetpar.2014.06.008 0304-4017/9 2014 Elsevier B.V. All rights reservedExplanation / Answer
1) The present study was conduted to study the prevalence of Dipylidum caninum in fleas of dogs and cats in Europe using the new PCR detection assay. the infection rate of Dipylidum in fleas obtained from cats and dogs were found to be 2.23% and 9.1% respectively and the difference was found to be statistically significant. The study is of observational cross sectional design and it has its own drawbacks. It is a snapshot of the condition prevailing at the time of study. The prevalence found in this study can be confirmed by performing follow up longitudinal study to establish the fact that the findings are not coincidental with the time of study and is same irrespective of the time and duration of the study.
2) The following changes can be made to the experiments described in the article.
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