Promoter fine mapping analysis is used to determine where the promoter starts an
ID: 92677 • Letter: P
Question
Promoter fine mapping analysis is used to determine where the promoter starts and ends in a gene by deleting regions of DNA adjacent to and within the gene. You have a putative gene which you subject to promoter fine mapping analysis. You observe that transcriptional activity is still maintained when deletion from the 5' end reaches nucleotide +53, but is lost upon further deletion past +53. Activity is also maintained when deletion from the 3' end reaches nucleotide +83, but is lost upon further deletion past +83. Please speculate what gene family this putative gene belongs to and state which RNA polymerase is used for its transcription. Promoter fine mapping analysis is used to determine where the promoter starts and ends in a gene by deleting regions of DNA adjacent to and within the gene. You have a putative gene which you subject to promoter fine mapping analysis. You observe that transcriptional activity is still maintained when deletion from the 5' end reaches nucleotide +53, but is lost upon further deletion past +53. Activity is also maintained when deletion from the 3' end reaches nucleotide +83, but is lost upon further deletion past +83. Please speculate what gene family this putative gene belongs to and state which RNA polymerase is used for its transcription.Explanation / Answer
Deletion of +53 to +83 regions destroys the transcriptional activity means the promoter binding site is located in that position which is inside the gene sequence. Thus these promoters are within the transcribe regions. This type of promoter generally found in case of transcription performed by pol III RNA polymerase enzyme and these polymerases are responsible for the transcription of tRNA and 5S rRNA in eukaryotes.
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