For each of the E. coli strains shown below (grown in the absence of glucose). I

Question

For each of the E. coli strains shown below (grown in the absence of glucose). Indicate whether the beta-galactosidase levels increase, remain constant, or are undetectable when the lac operon gratuitous inducer IPTG is added. Z^+ y^+ A increase B remain constant C undectable o^- z^+ y^+/I^- o^+ z y A undetectable B increase C remain constant You are studying a protein and wish to determine the nucleotide sequence that encodes its first two amino acids. The sequence of the first four amino acids of the normal protein is as follows: N-Met-Cys-Ser-Trp- -C Which one of the following is a possible mRNA sequence that could encode the first two amino acids? A ATGTGT B UACACA C GUACGU D AUGUGC E CGUGUA

Explanation / Answer

IPTG (Isopropyl-thio-Galactoside) is a fortuitous inducer. It  acetylates the 6’ CH2OH group so it induces

6’ acetyl, b 1’ isopropyl thio-Galactoside.

3.A increase

4.C remain constant.

binding of the inducer, IPTG, to the repressor affects the distribution of repressor between the two shapes. Thus, repressor bind with the inducer stabilized in the non-DNA-binding conformation.

5. D AUGUGC

AUG codes for met and UGC codes for cys.

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