A complex mixture of antioxidant polyphenolic flavonoids extracted from chocolat

Question

A complex mixture of antioxidant polyphenolic flavonoids extracted from chocolate was separated by normal phase HPLC using a bonded cyanopropyl column. Initial separation using on would best be and 50% acetonitrile (CH_3CN) provided insufficient resolution of the mixture improved by switching to Isocratic separation with 80% hexane and 20% acetonitrile. Isocratic separation with 20% hexane and 80% acetonitrile Gradient elution from 20% hexane 80% acetonitrile to 80% hexane/20% acetonitrile Gradient elution from 80% hexane/20% acetonitrile to 20% hexane /80% acetonitrile

Explanation / Answer

the correct option is B since it will allow better resolution of the mixture.

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