1.You have isolated a mutant that makes a temperature sensitive rho molecule; rh

Question

1.You have isolated a mutant that makes a temperature sensitive rho molecule; rho functions normally at 30 degree but not at 40 degree. If you grow this strain at both temperatures for a short period of time and isolate total, newly synthesized RNA, what relative size RNA do you expect to find for transcription units with rho-dependent terminators in each case?



2. Are the following E. coli cells constitutive or inducible for the z gene (of lac operon)?
e) i+ o+ z+
f) i- o+ z+
g) i- oc z+
h) i+ oc z+
i) is o+ z+

Explanation / Answer

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