In molecular cloning, most mammalian cells that are transfected with expression

Question

In molecular cloning, most mammalian cells that are transfected with expression vectors express the genes in the vector only transiently. Why (select all that apply)?

Question options:

because the vector cannot be stably passed on after cell division.

because transfection and/or expression may result in cell death.

because the vector is removed by restriction endonuclease.

because the foreign gene is integrated into the host chromosome.

because the vector cannot be stably passed on after cell division.

because transfection and/or expression may result in cell death.

because the vector is removed by restriction endonuclease.

because the foreign gene is integrated into the host chromosome.

Explanation / Answer

In molecular cloning, most mammalian cells that are transfected with expression vectors express the genes in the vector only transiently because the foreign gene is integrated into the host chromosome.

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