Make sure you label your plate correctly and include your unknown number. Incuba

Question

Make sure you label your plate correctly and include your unknown number. Incubate your plates in 37 degree C overnight to 48 hours. Performs a Gram stain on your unknown and records the results of the Gram reaction as well as the cell morphology below. Double check the Gram reaction and morphology with your TA by the end of the lab or the beginning of the next lab. 1. In the plaque assay, you must inoculate the soft agar with enough bacteria to produce a lawn of growth. Why is that important? 2. If you skipped the incubation time, the pre-adsorption phase, how could your results change? Why was the water bath set at 50-55 degree C for the soft agar? What might be some consequences of increasing or decreasing the temperature?

Explanation / Answer

Answer 1-

Virus are considered as neither living or dead cells. They are active or replicate only inside other host living cell. Phages are the type of viruses which replicate inside bacterial cells. Therefore in plaque assay, we need to first grow a lawn of bacterial cells, sufficient enough for viral particles to infect them and enter their genetic material for replication.

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