A solution containing tyrosine, glutamic acid and histidine was used in an elect
ID: 913403 • Letter: A
Question
A solution containing tyrosine, glutamic acid and histidine was used in an electrophoresis experiment. Match the description of the outcome for each of the chemicals to its name. The pH of the buffer solution used in the experiment was 5.63.
(?) Histidine (1) This amino acid will carry a net positive charge at pH 5.63 and so will migrate to the negative electrode
(?) Glutamic acid (2) This amino acid will bear no net charge at pH 5.63 and so will remain at the origin
(?) Tyrosine (3) This amino acid will carry a net negative charge at pH 5.63 and so will migrate to the positive electrode
Explanation / Answer
(1) This amino acid will carry a net positive charge at pH 5.63 and so will migrate to the negative electrode - Histidine
(2) This amino acid will bear no net charge at pH 5.63 and so will remain at the origin - Tyrosine
(3) This amino acid will carry a net negative charge at pH 5.63 and so will migrate to the positive electrode - Glutamic acid
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