An acid-base indicator, HIn, dissociates according to the following reaction in

Question

An acid-base indicator, HIn, dissociates according to the following reaction in an aqueous solution. The protonated form of the indicator, HIn, has a molar absorptivity of 3391 M–1·cm–1 and the deprotonated form, In–, has a molar absorptivity of 23230 M–1·cm–1 at 440 nm. The pH of a solution containing a mixture of HIn and In– is adjusted to 5.87. The total concentration of HIn and In– is 0.000156 M. The absorbance of this solution was measured at 440 nm in a 1.00 cm cuvette and was determined to be 0.830. Calculate pKa for HIn.

Explanation / Answer

An acid-base indicator, HIn, dissociates according to the following reaction in

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