Which of the following statements about the polymerase chain reaction (PCR) is f

Question

Which of the following statements about the polymerase chain reaction (PCR) is false?

(A) DNA amplified by PCR can be cloned.

(B) DNA is amplified at many points within a cellular genome.

(C) Newly synthesized DNA must be heat-denatured before the next round of DNA synthesis begins.

(D) The boundaries of the amplified DNA segment are determined by the synthetic oligonucleotides used to prime DNA synthesis.

(E) The technique is sufficiently sensitive that DNA sequences can be amplified from a single animal or human hair.

Explanation / Answer

Answer:-

Explanation: -option "B"-DNA is amplified at many points within a cellular genome.

1.Typically, a PCR reaction is performed to amplify the sequence of interest, and then it is joined to the vector via a blunt or single-base overhang ligation prior to transformation. Early PCR cloning often used Taq DNA Polymerase to amplify the gene. This results in a PCR product with a single template-independent base addition of an adenine (A) residue to the 3' end of the PCR product, through the normal action of the polymerase. These "A-tailed" products are then ligated to a complementary T-tailed vector using T4 DNA ligase, followed by transformation.

**Polymerase chain reaction (PCR) is a technique used in molecular biology to amplify a single copy or a few copies of a segment of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. It is an easy, cheap, and reliable way to repeatedly replicate a focused segment of DNA only.

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