A student researcher performed a chromatographic separation of caffeine and aspa

Question

A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, tc, was found to be 218.7 s with a baseline peak width, wc, of 13.7 s and the retention time for aspartame, ta, was 246.4 s with a baseline peak width, wa, of 21.7 s. The retention time for the unretained solvent methanol was 45.8 s. Calculate the average plate height in micrometers for this separation, given that it was performed on a 20.1- cm long column. Calculate the resolution, R, for this separation using the widths of the peaks. Calculate the resolution if the number of theoretical plates were to increase by a factor of 2.5.

Explanation / Answer

A student researcher performed a chromatographic separation of caffeine and aspa

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