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For most cells that have 0.9% NaCl in the cytoplasm, including E. coli , the (no

ID: 90690 • Letter: F

Question

For most cells that have 0.9% NaCl in the cytoplasm, including E. coli, the (non-0%) experimental environments used in the Osmotic Pressure lab are:

Select one:

a. Hyperosmotic

b. Acidic

c. Isosmotic

d. Hyposmotic

To increase turgor pressure in a culture of cells, you would add:

Select one:

a. Salt water (3% NaCl)

b. Saline (0.9% NaCl)

c. Distilled water

d. Extreme salinity (25% NaCl)

A common bacterial species living in the human intestine is:

Select one:

a. Staphylococcus aureus

b. Escherichia coli

c. Bacillus subtillis

d. Clostridium tetani

e. Streptococcus pyrogenes

In a solution where the solute concentration is high, the water concentration is:

Select one:

a. High

b. Low

c. Super duper pressurized

d. Unaffected

e. Equal

Which BSL does not require a primary barrier?

Select one:

a. BSL-1

b. BSL-2

c. BSL-3

d. BSL-4

e. BSL-5

If a non-infected individual tests positive for the infection, this would be

Select one:

a. A false negative

b. A false positive

c. A true negative

d. A true positive

e. High sensitivity

Explanation / Answer

1) Iso-osmotic environment would be used, because this would not cause any difference in the osmotic pressure in our desired pressure.

2) To induce turgour pressure, the cell cultures should be placed in distilled water, because it is hypotonic solution having low solute concentration. Because of low solute concentration and high water outside, all the water from outside moves inside the cells and fills the cells with water to an extent wherein it induces turgour pressure to the cell wall.

3) Common bacterial species living in human intestine is Escherichia coli.

4) In a solution where the solute concentration is high, the water concentration is low.

5) BSL-1 does not require a primary barrier. A BSL (biosafety level) is a set of biocontainment precautions required to isolate dangerous biological agents in an enclosed laboratory facility. The levels of containment range from the lowest biosafety level 1 (BSL-1) to the highest at level 4 (BSL-4).

6) If a non-infected individual tests positive for the infection, this would be false positive. which means the results shows that the person has infection but it is not the correct value, as the person doesn't have infection.