Fluorescence recovery after photobleaching (FRAP is a technique that allows visu

Question

Fluorescence recovery after photobleaching (FRAP is a technique that allows visualization of diffusion within the membrane. You set out to perform FRAP using four different samples of cells. In sample 1, you have fluorescently labeled a phospholipid. In samples 2, 3, and 4 you have fluorescently labeled membrane proteins X, Y, and Z, respectively. You photobleach an area of the membrane in each sample and record the rate of recovery of fluorescence. The data you obtain are shown in the graphs below: Using the data from these graphs, list the proteins in order of their ability to diffuse in the membrane, from fastest to slowest. Given the data on the graphs above, is the following statement a good hypothesis: protein X and protein Z are always present in the cell as part of the same protein complex? Explain your answer.

Explanation / Answer

A. The list of proteins in order of their ability to diffuse in the membrane from the fastest to the slowest.

Since the FRAP technique is used for the visualization of diffusion, as the fluorescence indicates the the amount of protein diffused. The large amount of fluorescence indicates more diffusion.

According to the graphs the large amount of fluorescence is observed in Protein X, Protein Z and Protein Y. So the fastest diffusion is done by protein X then Z and the slowest is protein Y.

B. According to the hypothesis protein X and Protein Z are always present in the cell as part of the same protein complex. Since the diffusion of protein X and Z are the proteins that diffuse in the cell. These proteins are diffuse almost at the same speed and same time. So they can b considered as the part of the same protein complex.

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