Mutant A: Lacl has a mutation in its DNA binding site such that it cannot bind t

Question

Mutant A: Lacl has a mutation in its DNA binding site such that it cannot bind the operator Mutant B: Mutation affects the protein that produces CAMP such that CAMP is produced at a high level all the time. Indicate the expression levels of beta-galactosidase in Mutant A and Mutant B Indicate with +, ++, or - only. +: Expression occurs (low level) ++: Expression occurs (high level) -: No expression almost no expression FYI: Wild type cells in the case of "neither glucose nor lactose" still produce a small amount of beta- galactosidase because Lacl is not always bound to the operator. Although Lacl stays bound to the operator most of the time, sometimes it falls off and allows transcription to take place for a short amount of time.

Explanation / Answer

Answer:

Based on the given information:

Expression levels of beta galactosidase in different conditions:

Will add explanation shortly.

Condition in cells Lactose only Glucose Only Glucose and Lactose Neither glucose nor lactose Wild type ++ - - + Mutant A + - + - Mutant B + ++ - -

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