How do you calculate mL to mg? How do you calculate the concentration of tyrosin

Question

How do you calculate mL to mg?

How do you calculate the concentration of tyrosinase in %(w/v) and mg/ml using the beer-lambert law?

For example the data given to us was

Reagent

Assay 1

Assay 2

Assay 3

Assay 4

Assay 5

Phosphate buffer (mL)

1.45

1.40

1.30

1.20

110

L-dopa (mL)

1.50

1.50

1.50

1.50

1.50

Tyrosinase (mL)

0.05

0.10

0.20

0.30

0.40

Reagent

Assay 1

Assay 2

Assay 3

Assay 4

Assay 5

Phosphate buffer (mL)

1.45

1.40

1.30

1.20

110

L-dopa (mL)

1.50

1.50

1.50

1.50

1.50

Tyrosinase (mL)

0.05

0.10

0.20

0.30

0.40

Explanation / Answer

Answer – For the converting of tyrosinase from mL to mg we must to know the density of tyrosinase.

We are given the volume of tyrosinase and we know the density of tyrosinase, so using the density formula we can calculate the mass of tyrosinase in gram from the given mL. Then we can convert this mass in gram to milligram.

We know formula for density

Density = mass / volume

Example -

Density of tyrosinase is like 1 g/L mens 0.001 g/mL

So for 1 mL it is 0.001 g

We know

1 g = 1000 mg

So, 0.001 g = ?

= 1 mg

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