Starting with equation (4.4) derive equation (4.5). Hint: write [Fe(CN) ] in ter

Question

Starting with equation (4.4) derive equation (4.5). Hint: write [Fe(CN) ] in terms of the formal concentration of glucose and the initial concentration of Fe(CN) and recognize that ‘d’ and ‘k’ are arbitrary constants.

In this experiment a series of solutions will be prepared containing a fixed initial concentration of Fe(CN_6^-3) and variable initial concentration of glucose. Under these conditions it can be shown that:

where ‘d’ and ‘f’ are constants and F is the “formal” concentration of glucose (i.e., the
concentration of glucose neglecting any reaction with Fe(CN_6^-3) ). In other words, the absorbance is
linearly dependent on the formal concentration of glucose. To analyse the glucose in the test solution
the same amount of Fe(CN_6^-3) is combined with a volume of a solution of unknown glucose
concentration and the absorbance is measured. Using equation (4.5), the concentration of the
glucose in the test solution can be calculated from the observed absorbance. This simpler method
(compared to the Trinder test) is used because the samples in this experiment will be simple aqueous
solutions as opposed to blood samples.

Explanation / Answer

A= eb [ Fe(CN)6-3]

the absorption depends on concentration of   [ Fe(CN)6-3]

[ Fe(CN)6-3] = [glucose ] initial - [Glucose ] final = [Glucose]F

So, plot abosrbance vs. F [glucose get linear plot

In this slope = k and intercept =m

A= d+k F glucose

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