When chromatigraphically purifiying LDH... How might you separate LDH from anoth

Question

When chromatigraphically purifiying LDH...

How might you separate LDH from another protein that has all of the same chemical characteristics as LDH (cellular location, solubility/hydrophilicity; magnitude of charge and can bind to NADH)?

a. Use a cation exchange resin instead of an anion exchange resin.

b. Use affinity chromatography with a resin composed of antibodies that are very specific for LDH.

c. There is nothing you can do to purifiy LDH away from this protein.

d. Use a higher percent of ammonium sulfate (~75%) to salt out the protein from LDH.

Explanation / Answer

The answer is b because the only factor that add specificity for LDH retention for the resine is the presence of antibodies that are specific for it.

Since the another protein has the same magnitude of change you can separate them using ionic exchange chromatography. Given that they have the same solubility, salting out can`t be made either.

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