PCB 3063 Fall 2005 Results and Discussion 1 Why were the restriction enzyme dige
ID: 89780 • Letter: P
Question
PCB 3063 Fall 2005 Results and Discussion 1 Why were the restriction enzyme digest done at 37 C 2. Hind III enzyme comes from Haemophilus Influenzae. What does the III in the name refer to 3,Would a Bam HI restriction Di of your suspect as a PstIdigest of it. Why or why DNA look the same Data Analysis 1. Examine your stained gel on a UV light box. Are the restriction fragments similar between the two samples? (suspect vs. crime scene) DNA we be certain that our committed the crime or that is to say that the matching restricted samples by our analysis our one and the 3. How do mutations in the DNA sequence affect this type of restriction enzyme analysis?Explanation / Answer
Ques-1:
The room temperature 37C of any lab, in which restriction enzymes are using for DNA digestion and cloning of required DNA. This is mainly useful to speed up the restriction digestion process to cut the selected molecular sites without any errors. In order to select a restriction enzyme, it is essential to read the nucleotide sequence of gene of interest and then identify the restriction enzyme sites. This process can be done appropriately at 37C based on these restriction enzyme sites, we will select the restriction enzymes because the restriction site is specific for each restriction enzyme. The question may be provided with a gene sequence, which is missing in the given information.
Ques-2:
Hind-III is the restriction enzyme, which is obtained from “Haemophilus influenza” of bacterial- strain D. The “III” represents 3rd isolated restriction n enzyme from the that above bacterial strain.
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