Proteases and chemicals which break specific bonds in peptide sequences: Chymotr

Question

Proteases and chemicals which break specific bonds in peptide sequences: Chymotrypsin, cuts on the C-terminal side of tyrosine, phenylalanine, and tryptophan. Trypsin, cuts on the C-terminal of lysine and arginine. Thermolyzing, cuts on the N-terminal side of leucine, phenylalanine, isoleucine, an valine. Pepsin, cuts on the C-terminal of phenylamine, leucine, and glutamic acid. Cyanogen bromide, cuts on the C-terminal side of methionine. Information about the amino acids in this sequence: Information about amino acids 1. All polar acidic amino acids are negatively charged 2. All polar basic amino acids are positively charged 3. One amino acid is polar with neutral side chain which has a phenol group on its side chain 4. An amino acid is non polar on the N terminus with only one carbon on its side chain 5. There is amino acid that is non- polar with 4- carbons on its side chain 6. There is a non-polar amino acid with no carbon on its side chain on the second position 7. An amino acid with basic side chain which is positively charged, contain an imidazole ring is on the fourth position of the sequence 8. An amino acid is on the C-terminus that is that has 2 carbons on its side chain and negative 9. There is an amino acid with acidic side chain which contain 3 nitrogen atoms on its side chain 10. Valine lies adjacent to a negatively charged amino acid which is on the C-terminus 11. One of the amino acids is non-polar with a benzene ring on its side chain. 12. Leucine lies close to a non chiral amino acid 13. A polar amino acid with S-H bond Experimental results A. Chymotrypsin digestion resulted in 2 pentapeptides of which one is positively charged and the other is neutral. B. Thermolyzing digestion resulted in a positively charged hexapeptide and a negative tetrapeptide C. Pepsin digestion resulted in a negative tripeptide and a positive heptapeptide D. Trypsin digestion resulted in positive hexapeptide and a negative tetrapeptide E. Thermolyzing digestion also results in a neutral dipeptide and a positive octapeptide F. Cyanogens bromide resulted in a decapeptide

Explanation / Answer

Ala- gly-leu-his- tyr -arg- phe- cys-val-Asp

If the side chain contains an acid functional group, the whole amino acid produces an acidic solution. The two acidic amino acids are aspartic and glutamic. If the side chain contains an amine functional group, the amino acid produces a basic solution.

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