You harvest mouse monocyte cell line raw 264.7 cells from a culture plate and de

Question

You harvest mouse monocyte cell line raw 264.7 cells from a culture plate and determine that you have a total volume of 9.6 ml. You remove an aliquot to a hemacytometer and count 239 cells in 1-mm square. You need to pass 1 X 10^6 cells to a new plate to maintain the culture. How would you do this? You harvest mouse monocyte cell line raw 264.7 cells from a culture plate and determine that you have a total volume of 9.6 ml. You remove an aliquot to a hemacytometer and count 239 cells in 1-mm square. You need to pass 1 X 10^6 cells to a new plate to maintain the culture. How would you do this?

Explanation / Answer

Tube 1 : Add 100 l of optimem + 1-2 l of LF2000.
Tube 2 : Add 100 l of optimem + 2 g of DNA.
Mix, incubate 20 minutes and add this to the cells with 1 mL optimen and then keep it overnight then change the media.We will find that the number of cells now would be 1 x 10^6 cells in the plate.

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