In a DNS reducing sugar assay of Diet Sprite, the absorbance is usually even low

Question

In a DNS reducing sugar assay of Diet Sprite, the absorbance is usually even lower than the absorbance of the water blank. Why might this be?

My initial thought was that because the DNS reaction is pH dependent (prefers alkaline conditions), the carbonation in diet sprite (which lowers the pH) might reduce the reactivity of DNS. Because in Diet Sprite, no reducing sugars (glucose/fructose) are present, DNS essentially converts nothing, and because of the pH effect of CO2, the absorption is driven even lower than 0. Am I correct?

Explanation / Answer

yes, diet sprite has no reducing sugars and the assay is done in alkaline conditions

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