Use the Michaelis-Menten equation to complete the enzyme kinetic data set, when

Question

Use the Michaelis-Menten equation to complete the enzyme kinetic data set, when K_m is known to have a value of 1 mmol/L. Fill-in-the-blank questions: Organic cofactors are referred to as The difference in values for Delta G and Delta G degree is in the One way to measure the rate of an enzymatic reaction is to measure the loss of over time. The Michaelis-Menten model assumes that is the rate constant ignored because P has not accumulated. The effect stabilizes the structure of DNA, resulting in more polar surfaces being exposed to the aqueous media.

Explanation / Answer

1. From Michaelis-Menten equation we have

KM = 1 mmol/L = 1 x 10-3 mol/L

For the 1st entry, v = 50 micromol L-1 min-1 = 50 x 10-6 mol L-1 min-1

and [S] = 0.5 mol L-1

Therefore vmax = v(1 + KM/[S]) = 50 x 10-6 mol L-1 min-1(1 + 1 x 10-3 mol L-1/0.5 mol L-1) = 50.1 x 10-6 mol L-1 min-1

Now putting vmax and [S] in the M-M equation we get the v for various [S]

For [S] = 1 mol L-1,

v = 50.1 x 10-6 mol L-1 min-1/ (1 + 1 x 10-3 mol L-1/1 mol L-1) = 50.04 x 10-6 mol L-1 min-1

For [S] = 2 mol L-1,

v = 50.1 x 10-6 mol L-1 min-1/ (1 + 1 x 10-3 mol L-1/2 mol L-1) = 50.07 x 10-6 mol L-1 min-1

For [S] = 3 mol L-1,

v = 50.1 x 10-6 mol L-1 min-1/ (1 + 1 x 10-3 mol L-1/3 mol L-1) = 50.08 x 10-6 mol L-1 min-1

For [S] = 10 mol L-1,

v = 50.1 x 10-6 mol L-1 min-1/ (1 + 1 x 10-3 mol L-1/10 mol L-1) = 50.09 x 10-6 mol L-1 min-1

2.

Related Questions

Navigate

• Browse (All)
• Browse U
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.