a plate that received 1000 microliters of a bacterial sample diluted by a factor

Question

a plate that received 1000 microliters of a bacterial sample diluted by a factor of 10^-6 had 298 colonies on it after incubation. What was the original cell density?

Please describe how to get to answer. Also please label answer. a plate that received 1000 microliters of a bacterial sample diluted by a factor of 10^-6 had 298 colonies on it after incubation. What was the original cell density?

Please describe how to get to answer. Also please label answer. a plate that received 1000 microliters of a bacterial sample diluted by a factor of 10^-6 had 298 colonies on it after incubation. What was the original cell density?

Please describe how to get to answer. Also please label answer.

Explanation / Answer

We will use the formula,

OCD = CFU/D x V

where,

OCD = original cell density

CFU = 298 CFU

D = 10^-6

V = 1000 microlitre = 1 ml

Feed values,

OCD = 298 CFU/10^-6 x 1 mL

= 298 x 10^6 CFU/ml

= 2.98 x 10^8 CFU/ml

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