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hello guy, can you help me find the concentration of [A] given these absorbance

ID: 889876 • Letter: H

Question




hello guy, can you help me find the concentration of [A] given these absorbance values and initial concentration

4. Prepare a table (No.1) as follows: 6.0 6.5 7.0 7.5 8.0 9.0 NA The phenolate ion concentration ([A]) can be calculated for each sample using Beer's r each samp le using Beer's law. Since the total concentration of p-nitrophenol and the phenolate ion remains constant, [HA] can be calculated as: [HA] 0.00004 M-[A] Show one set of example calculations for the determination of [A], [HA] and log [HAIIA Note: It's OK to get negative values for some log [AV[HA] values, especially at lower pH. This means that [HA]> [A], which we expect at lower pH a) Table numbered and titled (1 pt) b) Table itself (1 pt) c) One set of example calculations (1 pt) ndrson Hasselbach Equation

Explanation / Answer

Absorbance can be related always as follows:

C1/C2 = A1/A2

since we have the reference to be:

0.865 --> 0.04 we can relate all other quantities with C1/C2 = A1/A2 or more practical

A2 = A1*(C2/C1) = 0.04*C2/0.865 = 0.0462*C2 = A2

A2 = 0.0462*C2

0.715 --> 0.0462*0.715 = 0.033

0.52 --> 0.0462*0.52 = 0.024

0.365 --> 0.0462*0.365 = 0.0168

0.14 --> 0.0462*0.14 = 0.00647

0.133 --> 0.0462*0.133 = 0.006144