Which statement is FALSE regarding techniques of structure determination? X-ray
ID: 88843 • Letter: W
Question
Which statement is FALSE regarding techniques of structure determination? X-ray crystallography deduces the location of electrons in the crystal. NMR spectroscopy deduces the distances between certain atoms in the protein. Electron microscopy would be the best suited technique for obtaining atomic-level detail of structure in small, highly flexible proteins. Prior knowledge of the sequence of the protein of interest is important for the success of a structure determination experiment. All of the above are true.Explanation / Answer
Answer:
A3. Electron microscopy would be the best suited technique for obtaining atomic-level detail of structure in small, highly flexible proteins.
(THIS IS THE FALSE STATEMENT)
Justification:
As the materials that can be viewed in an electron microscope generally require processing to produce a suitable sample, it is not at all suitable for obtaining atomic-level detail of structure in small, highly flexible proteins. Because, the inside of an electron microscope is under high vacuum in order to enable the electron beam to travel in straight lines.
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