Gradebook 10/8/2015 08:00 AM 6.7/9 CalculatorPeriodic Table Print Question 7 of

Question

Gradebook 10/8/2015 08:00 AM 6.7/9 CalculatorPeriodic Table Print Question 7 of 9 Map dt sapling eaming A DNA fragment was sequenced using dideoxy DNA sequencing. In this procedure, ddATP was tagged with a green dye, ddCTP was tagged with a blue dye, ddGTP was tagged with a yellow dye, and ddTTP was tagged with a red dye. The primer used had the sequence 5'-ATGAC-3'. Given the gel electrophoresis representation shown below, what was the sequence of the DNA fragment (template strand)? Step 1: The nucleotide sequence of the newly synthesized DNA is 3- CGCTATCTTTTCCGG-5: Step 2: The nucleotide sequence, including the primer, is 3-COCTATCTTTTCCGGCAGTA-5' Step 3: Write the complementary (template) nucleotide sequence 3' O Previous Give Up & View Solution Q) Check Answer 0 Next Exit- about us careers partners privacy policy terms of use contact us

Explanation / Answer

Base:
CGCTATCTTTTCCGCAGTA

complement of C is G
complement of T is A
complement of G is C
complement of A is T

so complement base is:
GCGTTAGAAAAGGCGTCAT

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