Which feature is NOT a requirement of the procedure for determining a protein\'s

Question

Which feature is NOT a requirement of the procedure for determining a protein's structure by X-ray crystallography? The crystal must diffract an X-ray beam to produce a diffraction pattern of spots. The preferred bond lengths of atoms in amino acids and the electron density map of the protein must be already known to interpret the experimental data from X-ray crystallography. The protein molecules must assemble into a crystal where the molecules are aligned in the same orientation. The spots in the diffraction pattern must be observed to deduce the electron density map in the crystal. All of the above are required.

Explanation / Answer

Answer: Option A2.

X-Ray diffraction results are in the terms of electron density and bond length, therefore the prior knowledge of these is not required.

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