TECHNIQUE: Fractionation by solubility (Centrifugation)) The procedure for purif
ID: 88827 • Letter: T
Question
TECHNIQUE: Fractionation by solubility (Centrifugation)) The procedure for purifying a certain enzyme calls for centrifugation of the homogenate, then adding ammonium sulfate to the supernatant, and then a second centrifugation with the supernatant being retained and subsequently dialyzed. Which of the following statements is most likely TRUE? The enzyme of interest precipitated when ammonium sulfate was added. A benefit of dialysis is to remove impurities like chromosomal DNA. Cellular organelles like mitochondria would be removed in the first centrifugation pellet. Enzyme assays cannot be performed until after the dialysis is completed. All of the above are false.Explanation / Answer
Answer:
Technique: Fractionation by solubility
The procedure for purifying a certain enzyme calls for centrifugation of the homogenate, then adding ammonium sulfate to the supernatant, and then a second centrifugation with the supernatant being retained and subsequently dialyzed. Which of the following statements is most likely true?
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