The criteria for acceptable biological and biochemical buffers were established

Question

The criteria for acceptable biological and biochemical buffers were established by studies conducted by Norman Good et al. in 1966. These criteria included

•a pKa within physiological range

•solubility in water

•membrane impermeability

•minimal salt effect or ion interactions

•minimal temperature and concentration effects on dissociation

•chemical stability

•no/minimal, or well-documented, UV light absorbance at certain wavelengths

•easy to prepare Choose the buffer characteristic, if any, that fails to meet the criteria. Choose the best answer listed.

HINT: Physiological pH is 7.4.

Mapdab The criteria for acceptable biological and biochemical buffers were established by studies conducted by Norman Good et al. in 1966. These criteria included a pKa within physiological range .solubility in water membrane impermeabilit minimal salt effect or ion interactions minimal temperature and concentration effects on dissociation .chemical stability no/minimal, or well-documented, UV light absorbance at certain wavelengths .easy to prepare Choose the buffer characteristic, if any, that fails to meet the criteria. Choose the best answer listed O Nat HN pKa at 25 °C = 7.48 lpKarc =-0.0 HEPES pK2 at 25 -720 ApKa/ C-0.0028 may react with calcium or magnesium salts may inhibit some enzymes Phosphate buffer KaC0.014 may interfere with a type of protein assay low absorbance in UV range ineffective buffer near physiological pH O O ineffective buffer near physiological pH minimal solubility in water influence of temperature on minimal solubility in water O soluble in nonpolar solvents O unknown UV light absorbance 0 none of the above Scroll down dissociation is too high interactions with cations none of the above see all options O O

Explanation / Answer

phosphate buffer :   interaction with ctions

HEPES :      none of the above

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