Explain your answer to the previous question. Briefly describe one reason for ad

Question

Explain your answer to the previous question. Briefly describe one reason for adding loading dye (a.k.a. loading buffer) to your DNA sample before you place the DNA sample into your gel. If you find that your gel is secured into the casting tray with tape, you should (remove or leave in place) the tape before running electrical current through the gel. The red end of a gel box represents the positively-charged end. Based on this, you want to position your gel so that the DNA samples will travel towards the (red or black) end. You should pour the running (directly on top of or just to the side) of the gel. Explain your answer to the previous question. Its better to leave the box of pipette tips (closed or open) throughout the entire lab period. Describe two things you can do to stabilize yourself (and the micropipette) as youre loading your sample into your gel. After loading your sample into a well, when should you let go of the plunger on your micropipette?

Explanation / Answer

(3) By adding loading dye to the DNA sample, you will make the DNA sample to sink into the wells of the gel so that you can track the DNA sample while it is migrating through the gel. Another reason is loading dye will give color to the DNA, so that you can see them with your naked eyes.

(4) If you find that your gel is secured into the casting tray with tape, you should remove the tape before running an electric current through the gel.

You need to remove the tape from the ends of the casting tray before keeping the gel in the electrophoresis chamber so that the 1X TAE buffer can pass through this.

(5)The red end of a gel box represents the positively-charged end. Based on this, you want to position your gel so that the DNA samples will travel towards the red end.

You will be orienting your gel so that the wells will be near the negative (black) terminal, so obviously, the DNA sample will travel towards the red end (positive end).

(6) You should pour the running buffer just to the side of the gel.

(7) If you pour running buffer on top of the gel, it will disturb the DNA sample loaded in the wells and the DNA migration in the gel will not be proper.
(8)It’s better to leave the box of pipette tips closed throughout the entire lab period.

Otherwise, dust will enter into the pipette tip box

(9)Either you try to stabilize the pipett with a finger using your other hand
You can even rest your elbows on the bench top and try to use both hands.

(10) After completely removing your micropipette from the gel chamber, otherwise, it will suck the sample again and will disturb the setup.

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