1. In a theoretical laboratory, students are studying the protein synthesis path
ID: 88388 • Letter: 1
Question
1. In a theoretical laboratory, students are studying the protein synthesis pathway: DNAmRNAprotein. During observation at several work stations, they record some discrepancies in the final protein production. Their observation notes are found below.
Laboratory Report
Student 1
Student 2
Student 3
Observation 1
Non-coding mRNA segments are present.
Observation 2
DNA remains intact. No unzipping of helical structure.
Observation 3
Polypeptide chains vary in length.
(a) Predict the potential cause of Observation 1.
(b) Discuss some of the potential cause of Observation 2 for gene expression.
(c) Provide a rationale for Observation 3.
Laboratory Report
Student 1
Student 2
Student 3
Observation 1
Non-coding mRNA segments are present.
Observation 2
DNA remains intact. No unzipping of helical structure.
Observation 3
Polypeptide chains vary in length.
(9 points) 6. In a theoretical laboratory, students are studying the protein synthesis pathway: DNAweuRNAweprotein. During observation at several work stations they record some discrepancies in the final protein production. Their observation notes are found below. Laboratory Report Student 1 Student 2 Student 3 Observation Non-coding mRNA segments are present. Observation DNA remains intact. No unzipping of helical structure Observation Polypeptide chains vary in length. (a) Predict the potential cause of Observation 1. (b) Discuss some of the potential cause of Observation 2 for gene expression (c) Provide a rationale for observation 3.Explanation / Answer
Ans a: The Non coding segments of mRNA suggest the presence of introns which may be due to defect in the splicing machinery that operates post-transcriptionally.
Ans b: The unzipping of DNA not occuring in second case owes a reason to the absence to helicase or can due to absence of single strand DNA binding proteins (SSBs).
Ans c : in the third scenario the result may be due to various splicing varients generated during post transcriptional modification.
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