How can a mutation of a single base pair affect gene expression? Here’s an examp

Question

How can a mutation of a single base pair affect gene expression? Here’s an example: The DNA used above was mutated during replication prior to cell division. Here is the original strand and the mutant strand. Will their protein products be different from one another? If so, how? Show your work

Original DNA:   TACCAAAAAGGGCGAACAAATAAAATT

Mutant DNA:     TACCAAAAAGGGCGAACTAATAAAATT

. What is the mRNA sequence resulting from transcription of the gene below?

DNA:      TACCAAAAAGGGCGAACAAATAAAATT

mRNA: 'What sequence of tRNA results from complementary base pairing with the mRNA sequence above?

Define translation in your own words.

Explanation / Answer

Original DNA:   TACCAAAAAGGGCGAACAAATAAAATT

Mutant DNA:     TACCAAAAAGGGCGAACTAATAAAATT (mutation shown with bold letter)

mRNA: AUG GUU UUU CCC GCU UGA UUA UUU UAA

protein : met-val-phe-pro-ala-stop

tRNA: UAC CAA AAA GGG CGA ACU

Translation: It is the process by which protein synthesis takes place from mRNA strand with the help of ribosomes. It is followed by transcription of RNA from DNA.

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