There is an outbreak of Staphylococcus aureus food poisoning in the Salem. OR ar

Question

There is an outbreak of Staphylococcus aureus food poisoning in the Salem. OR area. A common factor in all the hospital cases is that people affected with symptoms said they had eaten at the Popeyes Louisiana kitchen and that all of them had ordered the coleslaw with their delicious chicken meals. What techniques and procedures learned in class could you use to determine if there is S. aureus contaminating the coleslaw? Three different workers were responsible for preparing the coleslaw during business hours. Jill prepared the coleslaw in the morning, Steve prepared the coleslaw in the afternoon and Jack made the coleslaw in the evening. Using the information below. A) Calculate the Staph CFU/gram of coleslaw for each sample. B) determine who might have contaminated the coleslaw. 1 gram of coleslaw was used from each sample. Bonus: A bioengineer is running fermentation experiments to produce a new biofuel. The biofuel is produced as a byproduct of normal metabolism of lactose by a newly identified fecal coliform bacterial species. Currently the yield of biofuel is half the expected amount. The engineer suspects that there may be another bacterial species contaminating the recent fermentation. Use the information below to answer the following:

Explanation / Answer

PART 1

-Coleslaw is a side dish mostly served with chicken meals and barbeque chicken majorly made using raw shredded cabbage and vinaigrette salad dressing.

-S.aureus can produce a range of extracellular enzymes some can be toxins and other chemical components.

A specific test that can be performed is coagulase test and TNase test as they are capable of producing coagulase enzyme that can coagulate plasma and thermonuclease TNase a thermostable phosphodiesterase that can cleave DNA or RNA.

The S.aureus colonies can be detected and isolated using Azide Blood agar medium too.

PART 2:

-It can be concluded using the given data for the no. of colonies that the meal prepared by Steve had a contamination rate more than thay prepared by both Jack and Jill.

Bonus:

An EMB i.e Eosin Methylene Blue agar plating was performed by the bioengineer to check out for the presence of the contaminating fecal species, it gave a green metallic sheen which is observed in case of E.coli species and hemolysis test results also showed the presence of gran negative bacilli thus confirming the contamination of the medium by E.coli

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