answer all the qustions ( you have to be sure ) 1- When using the light microsco

Question

answer all the qustions ( you have to be sure )

1- When using the light microscope:

A- You always start with a high power and work your way down in magnification because the microscopes we have are parfocal

B- You always use immersion oil with the 40X objective to get the best image

C- Organisms are usually stained when using brightfield microscopy

D- The advantage of darkfield is being able to view live, unstained (and sometimes motile) cells

2- The negative stain is used to

A- Visualize Endospores.

B- Determine Gram Reaction.

C- Determine Flagella Arrangement.

D- Visualize Capsules

E- Determine cell size.

3- Select all that apply. Koch and Pasteur collaborated in the late 1800s to:

A- Link specific microorganisms with specific diseases

B-Develop sterilizing methods for laboratory supplies.

C- Develop a method for using agar to solidify nutrient/solid plates

D- Confirm that some microbes cause disease

4-

All organisms used for study in the lab are to be handled as though they were:

A- Ebola

B- Harmless

C- Potential pathogens

5-

Select all that apply. Microorganisms play a role in

A- Disease.

B- Biodegradation.

C- Cheese Production.

D- Nitrogen Recycling.

A- You always start with a high power and work your way down in magnification because the microscopes we have are parfocal

B- You always use immersion oil with the 40X objective to get the best image

C- Organisms are usually stained when using brightfield microscopy

D- The advantage of darkfield is being able to view live, unstained (and sometimes motile) cells

Explanation / Answer

ANS1. C (Organisms are usually stained when using brightfield microscopy) and D ( The advantage of darkfield is being able to view live, unstained and sometimes motile cells),

ANS2:Negative staining used to D- Visualize Capsules,

E- Determine cell siz

C- Determine Flagella Arrangement.

ANS3:A- Link specific microorganisms with specific diseases.(cause of many disease where identified like cholera,plaque,tuberculosis).

ANS4:C-Potential pathogens.

ANS5:ALL OF THE ABOVE.

C- Determine Flagella Arrangement.

ANS3:A- Link specific microorganisms with specific diseases.(cause of many disease where identified like cholera,plaque,tuberculosis).

ANS4:C-Potential pathogens.

ANS5:ALL OF THE ABOVE.

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