Alizarin, sudan III, and bromocresol green are types of dyes used for staining a

Question

Alizarin, sudan III, and bromocresol green are types of dyes used for staining a variety of materials, ranging from clothing to triglycerides. A clever criminal has mixed all three of them together and used the mixture to make it difficult to analyze the clothing and DNA left behind at a crime scene. Find the structures and the acidity (pKa) of each dye and propose a method for separating them using the extraction technique learned in a lab experiment. You will need to identify three specific solvents, two aqueous and one organic. (Hint: the acid-base properties of the dyes and solvents is the key to solving this problem)

Explanation / Answer

Summary We developed a rapid detection method for Legionella pneumophila (Lp) by filtration, immunomagnetic separation, double fluorescent staining, and flow cytometry (IMS?FCM method). The method requires 120?min and can discriminate

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