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The isoelectric point, pl, of the protein chymotrypsinogen A is 9.1 , while that

ID: 875689 • Letter: T

Question

The isoelectric point, pl, of the protein chymotrypsinogen A is 9.1 , while that of yeast alcohol dehydrogenase is 5.4. What is the net charge of chymotrypsinogen A at pH 5.1? ____ What is the net charge of yeast alcohol dehydrogenase at pH 7.5? _______ The isoelectric point of methionine is 5.74 ; aspartic acid , 2.77. During paper electrophoresis at pH 6.5 , toward which electrode does methionine migrate? During paper electrophoresis at pH 4.5 , toward which electrode does aspartic acid migrate?

Explanation / Answer

net charge on chymotrpsin at pH 5.1 = 0.4

net charge of yeast alcohol dehydrogenase at pH 7.5 = 0.5

At pH 6.5 methionine will migrate towards cathode

At pH 4.5 aspartic acid will mirate towards anode

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